Liquid chromatography-mass spectrometry platform for both small neurotransmitters and neuropeptides in blood, with automatic and robust solid phase extraction

Liquid chromatography-mass spectrometry platform for both small neurotransmitters and neuropeptides in blood, with automatic and robust solid phase extraction

Neurons communicate via chemical signals called neurotransmitters (NTs). The numerous identified NTs can have very different physiochemical properties (solubility, charge, size etc.), so quantification of the various NT classes traditionally requires several analytical platforms/methodologies. We here report that a diverse range of NTs, e.g. peptides oxytocin and vasopressin, monoamines adrenal...

Extraction and Detection of Pentobarbital in Soil by Solid Phase Extraction and Liquid Chromatography / Mass Spectrometry

ON-LINE SOLID-PHASE EXTRACTION AND LIQUID CHROMATOGRAPHY/PARTICLE BEAM-MASS SPECTROMETRY FOR DEGRADATION STUDIES OF SOME POLAR PESTICIDES IN WATER

An on-line automated method for photodegradation studies of isoproturon, diuron, atrazine, fenitrothion, and metoxuron by means of liquid chromatography/mass spectrometry (LC/MS) with particle beam (PB) interface is described. Surface water samples were first spiked with 50 µg/l of each pesticide and then exposed to the radiation of the medium-pressure mercury lamp. Next, in regular intervals o...

Analysis of Digoxin and Metildigoxin in Whole Blood Using Solid-Phase Extraction and Liquid Chromatography Tandem Mass Spectrometry

A simple and rapid UPLC/MS/MS method has been developed and validated for the analysis of digoxin and metildigoxin in whole blood. Samples were prepared by SPE extraction with Oasis HLB columns. Separation was achieved with an ACQUITY UPLC HSS T3 column (2.1 × 100; 1.8 μm), at 35°C. The mobile phase consisted of acetonitrile (70%) and ammonium formate 5 mM (30%). Total run time was 1.5 min oper...

Solid-phase extraction and analysis of THC and carboxy-THC from whole blood using a novel fluorinated solid-phase extraction sorbent and fast liquid chromatography-tandem mass spectrometry.

In this study, solid-phase extraction (SPE) is described using a novel fluorinated [heptadecafluorotetrahydrodecyl (C(10)H(4)F(17))] phase to isolate THC and its primary metabolite carboxy-THC from whole blood samples. SPE was performed in hydrophobic mode after samples of whole blood were precipitated with acetonitrile. After applying the sample to the SPE column in aqueous phosphate buffer (p...